Malware analysts often strip signatures to study how a file behaves without the "trusted" status granted by a certificate.

There are several legitimate and technical reasons why someone might look for a way to unsign a file:

If you are working on a specific project, I can provide more detail if you tell me: What are you targeting? Are you getting a specific error code (e.g., 0x800b0100)? Is this for personal research or software deployment ?

This article explores how the Windows SignTool works, the implications of unsigning software, and the technical methods used to remove digital signatures. Understanding Digital Signatures and SignTool

Right-click and select "Delete" or set the Size and Address values to zero. 3. Using PowerShell

If an old internal tool has a certificate from a defunct CA (Certificate Authority), it may cause hang-ups on modern systems. Methods to Unsign Executables